Coding

Part:BBa_K3656307:Design

Designed by: Huang feiyu   Group: iGEM20_AHUT-ZJU-China   (2020-10-23)


OT3-Carbonic Anhydrase-Mutant-His


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 470
    Illegal XhoI site found at 520
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

none.


Source

This is an improved part of mutant carbonic anhydrase from Pyrococcus horikoshii OT3,with a His-tag to the C-terminal of the CDS that is used for purification of carbonic anhydrase protein. We use pET28(a+) to express protein efficiently.

References