Coding
Part:BBa_K3656307:Design
Designed by: Huang feiyu Group: iGEM20_AHUT-ZJU-China (2020-10-23)
OT3-Carbonic Anhydrase-Mutant-His
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 470
Illegal XhoI site found at 520 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
none.
Source
This is an improved part of mutant carbonic anhydrase from Pyrococcus horikoshii OT3,with a His-tag to the C-terminal of the CDS that is used for purification of carbonic anhydrase protein. We use pET28(a+) to express protein efficiently.